02772naa a2200229 a 450000100080000000500110000800800410001902400390006010000200009924501250011926000090024452020680025365000160232170000200233770000200235770000220237770000200239970000130241970000170243270000250244977300680247419173382012-03-02       2011    bl ---       0--  u    #d7 a10.1016/j.soilbio.2010.09.0192DOI1 aNAKATANI, A. S.  aChanges in the genetic structure of Bacteria and microbial activity in an agricultural soil amended with tannery sludge.  c2011  aThe application of tannery sludge to soils is a form of recycling; however, few studies have examined the impacts of this practice on soil microbial properties. We studied effects of two applications (2006 and 2007) of tannery sludge (with a low chromium content) on the structure of the bacterial community and on the microbial activity of soils. We fertilized an agricultural area in Rolândia, Paraná state, Brazil with different doses of sludge based on total N content, which ranged from 0 to 1200 kg N ha1. Sludge remained on the soil surface for three months before being plowed. Soils were sampled seven times during the experiment. Bacterial community structure, assessed by denaturing gradient gel electrophoresis (DGGE), was modified by the application of tannery sludge. Soon after the first application, there was clear separation between the bacterial communities in different treatments, such that each dose of sludge was associated with a specific community. These differences remained until 300 days after application and also after the second sludge application, but 666 days after the beginning of the experiment no differences were found in the bacterial communities of the lowest doses and the control. The principal response curve (PRC) analysis showed that the first sludge application strongly stimulated biological activity even 300 days after application. The second application also stimulated activity, but at a lower magnitude and for a shorter time, given that 260 days after the second application there was no difference in biological activity among treatments. PRC also showed that the properties most influenced by the application of tannery sludge were enzymatic activities related to N cycling (asparaginase and urease). The redundancy analysis (RDA) showed that tannery sludge?s influence on microbial activity is mainly related to increases in inorganic N and soil pH. Results showed that changes in the structure of the bacterial community in the studied soils were directly related to changes of their biological activity.  aNitrogênio1 aMARTINES, A. M.1 aNOGUEIRA, M. A.1 aFAGOTTI, D. S. L.1 aOLIVEIRA, A. G.1 aBINI, D.1 aSOUSA, J. P.1 aCARDOSO, E. J. B. N.  tSoil Biology & Biochemistrygv. 43, n.1, p. 106-114, Jan. 2011.