02062naa a2200229 a 450000100080000000500110000800800410001902400540006010000260011424502400014026000090038052012160038965000230160565000310162865300160165970000230167570000130169870000250171170000170173670000160175377300630176919093092024-02-05       2012    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.vetpar.2011.10.0072DOI1 aMONTEIRO, C. M. de O.  aHeterorhabditis bacteriophora (RhabditidabHeterorhabditidae) HP88 for biological control of Rhipicephalus microplus (Acari: Ixodidae): the effect of different exposure times of engorged females to the nematodes.h[electronic resource]  c2012  aThe aim of this study was to assess the influence of different exposure times of engorged female the Rhipicephalus microplus to infective juveniles of Heterorhabditis bacteriophora isolate HP88. The engorged females were divided into seven groups (six treatments and one control) of 20 ticks each with statistically similar average weights (p &gt 0.05) and exposed to concentrations of 300 nematodes/tick for periods of 3, 6, 12, 24, 48 and 72 h. The following biological parameters were monitored: pre-oviposition period; egg mass weight; hatching percentage; and efficacy of treatment. There was no influence of the exposure time on the pre-oviposition period (p &gt 0.05), while there were significant differences (p &lt 0.05) between the groups exposed for 24, 48 and 72 h and the control group with respect to the egg mass weight, and 24 h for hatching percentage. Treatment efficacy reached 100% after exposure for 48 and 72 h. These results demonstrate that infective juveniles of H. bacteriophora HP88, under laboratory conditions, interfere in the majority of the biological parameters of the non-parasitic phase of engorged R. microplus females when the exposure time is greater than or equal to 24 h.  abiological control  aentomopathogenic nematodes  aCattle tick1 aPRATA, M. C. de A.1 aFAZA, A.1 aBATISTA, E. S. de P.1 aDOLINSKI, C.1 aFURLONG, J.  tVeterinary Parasitologygv. 185, n. 3/4, p. 364-367, 2012.