01938naa a2200193 a 450000100080000000500110000800800410001910000220006024500870008226000090016952013720017865000200155065300210157070000260159170000160161770000210163370000230165477300670167719026162011-10-06       2011    bl uuuu     u00u1 u    #d1 aREZENDE, J. C. de  aCalli induction in leaf explants of coffee elite genotypes.h[electronic resource]  c2011  aThree experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.  aCoffea Arábica  aGrowh regulators1 aCARVALHO, C. H. S. de1 aPASQUAL, M.1 aSANTOS, A. C. R.1 aCARVALHO, S. M. de  tCiência Rural, Santa Mariagv.41, n.3, p. 384-389, mar, 2011.