01690naa a2200301 a 450000100080000000500110000800800410001902200140006002400410007410000290011524501150014426000090025950000800026852007310034865000140107965000230109365000220111665000220113865000110116065300100117165300180118165300210119970000220122070000260124270000250126870000190129377300760131218961142011-07-27 2011 bl uuuu u00u1 u #d a1982-56767 a10.1590/S1982-567620110002000092DOI1 aBESERRA JUNIOR, J. E. A. aSequence variability in the coat protein gene of cowpea severe mosaic virus isolates from northeastern Brazil. c2011 aRosa F. R. Araújo Camarço [i.e. Rosa Felícia Escóssia Araújo Camarço] aThe comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates. aComovirus asequence diversity aDoença de Planta aVigna Unguiculata aVírus aCPSMV aFeijão-caupi aFeijão-de-corda1 aANDRADE, E. C. de1 aCAMARÇO, R. F. R. A.1 aNASCIMENTO, A. K. Q.1 aLIMA, J. A. A. tTropical Plant Pathology, Brasília, DFgv. 36, n. 2, p. 121-124, 2011.