01444nam a2200241 a 450000100080000000500110000800800410001910000170006024500870007726001510016430000140031552006850032965000120101465000230102665000240104970000190107370000170109270000230110970000190113270000140115170000170116570000200118218889412013-09-20       2011    bl uuuu     u00u1 u    #d1 aDINON, A. Z.  aPrimer development for detection of Phaseolus vulgaris and Olathe transgenic bean.  aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Ribeirão Preto: Sociedade Brasileira de Genéticac2011  c1 CD-ROM.  aSeveral strategies have been employed for genetically engineering resistance to viruses in transgenic plants. The bean golden mosaic virus (BGMV) is responsible for causing the gold mosaic of common bean (Phaseolus vulgaris L.) and causes severe yield losses due to yellow-green mosaic of leaves, stunted growth and distorted pods. Olathe transgenic bean (Olathe 5.1) was successfully produced by EMBRAPA using the RNA interference (RNAi) concept to silence the rep viral gene in common bean to generate transgenic lines with strong resistance to BGMV. In this work, real time PCR (RT-PCR) method with SYBR Green was developed to detect this newly genetically modified (GM) plant.  aFeijão  aPhaseolus vulgaris  aPlanta transgênica1 aBROD, F. C. A.1 aMELLO, C. S.1 aSILVEIRA, J. C. M.1 aKOLLING, D. J.1 aTREML, D.1 aFARIA, J. C.1 aARISI, A. C. M.