02001naa a2200241 a 450000100080000000500110000800800410001910000200006024500870008026000090016750001180017652011690029465000210146365000230148465000270150765000220153465000240155665000280158065300230160870000220163170000140165377300920166718836712018-05-10       2010    bl uuuu     u00u1 u    #d1 aLANNA FILHO, R.  aBacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants.  c2010  aTítulo em português: Biocontrole da mancha?bacteriana e da pinta?preta por bactérias epifíticas em tomateiro.  aThe objective of this work was to evaluate in vitro and in vivo biocontrol of Xanthomonas vesicatoria) Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS). In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens.  aBacillus pumilus  abiological control  aPaenibacillus macerans  aAlternaria Solani  aControle Biológico  aXanthomonas Vesicatoria  aEpiphytic bacteria1 aROMEIRO, R. da S.1 aALVES, E.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 45, n. 12, p. 1381-1387, dez. 2010