02637nam a2200217 a 450000100080000000500110000800800410001910000200006024500900008026001570017050000140032752019150034165300280225665300190228465300140230365300140231770000200233170000200235170000280237170000200239918831332011-04-29       2011    bl uuuu     u00u1 u    #d1 aOLIVEIRA, T. M.  aIdentification of proteins involved in response mechanisms to water stress in citrus.  aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.c2011  apdf 34710  aThe citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism and production of energy, proteins related to metabolism of carbohydrates and proteins unknown biological function. Identifying these proteins will help in understanding of the biochemical processes involved in the mechanisms of response to drought and provide input to the development of rootstocks drought tolerant, so use this information to improve this important group of plants through genetic manipulation.  aDifferential expression  aDrought stress  aProteomic  aRootstock1 aPIROVANI, C. P.1 aCOSTA, M. G. C.1 aSOARES FILHO, W. dos S.1 aGESTEIRA, A. S.