01626nam a2200229 a 450000100080000000500110000800800410001910000190006024501610007926001520024050000170039252008180040965000160122765000110124365300230125470000220127770000190129970000250131870000140134370000200135770000190137718831092023-06-05 2010 bl uuuu u00u1 u #d1 aNOVELLI, V. M. aPrevalence of the Cardinium symbiont and genetic variability of Brevipalpus phoenicis (AcaribTenuipalpidae) populations from Brazil.h[electronic resource] aIn:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 181-182.c2010 aAbstract 335 aThe presence of Cardinium symbiont has been reported and is associated with feminization in Brevipalpus mites and alterations of the reproduction of several other arthropod hosts. We report the prevalence of this endosymbiont and genetic variability of B. phoenicis populations from different plant hosts and geographic regions of Brazil. The presence of bacteria was confirmed by PCR amplification and transmission electron microscopy, and their variability was evaluated by analysis of the 16S rDNA and gyrB gene region. For B. phoenicis variability, we evaluated mitochondrial COI sequences. High similarity was observed between Cardinium from different mite populations, and interestingly, although the symbiont has been associated with the haploid thelytoky, two mite populations were naturally aposymbiotic. aBrevipalpus aƁcaro aCardinium symbiont1 aFREITAS-ASTUA, J.1 aRIBEIRO, B. M.1 aLOCALI-FABRIS, E. C.1 aNAVIA, D.1 aKITAJIMA, E. W.1 aMACHADO, M. A.