02673nam a2200229 a 450000100080000000500110000800800410001910000170006024500940007726001440017150001040031552018350041965000220225465300190227665300300229565300180232570000170234370000250236070000210238570000180240670000190242418727472011-01-19       2010    bl uuuu     u00u1 u    #d1 aCUNHA, B. A.  aDifferent symptomatic tissues as sources of inoculum to citrus leprosis virus c (CILV-C).  aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.c2010  a128-PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010  aCitrus leprosis virus C (CiLV-C), transmitted by Brevipalpus sp. (Acari: Tenuipalpidae), is the causal agent of citrus leprosis, a disease that causes estimated annual losses of US$ 100 million in Brazilian orchards. Studies showed that the vector has no ability to transmit the virus to their offspring and needs to feed on infected tissue for the CiLV-C acquisition. Furthermore, the virus does not invade the host systemically, remaining inside the localized lesions it induces in infected tissues. These two features significantly increase the importance of the presence of the inoculum in the orchard, and pruning of symptomatic branches have been recommended as a way to reduce the source of inoculum in the field. However, there is no consistent information on the relative importance of different plant tissues or age of lesions as sources of inoculum. In this study, we evaluated the colonization and multiplication of the mites in branches of different diameters, and the efficiency of these branches, as well as leaves with chlorotic or necrotic lesions as sources of CiLV-C inoculum. A clonal population of B. phoenicis was maintained onto the different tissues for 96 h for viral acquisition. Part of the viruliferous mites were transferred to seedlings of Pera sweet orange (Citrus sinensis L. Osbeck), highly susceptible to CiLV-C, while others were submitted to RT-PCR to confirm the presence of the virus. After two weeks, large numbers of eggs and nymphs were observed on the branches, suggesting efficient colonization from the mites. Leprosis symptoms were observed in citrus seedlings infested with mites maintained in leaves and branches as sources of inoculum. Our data suggest that CiLV-C is present in both tissues and different types of lesions, which may play a role as sources of inoculum in the field.  aDoença de Planta  aBrevipalpus sp  aCitrus sinensis L. Osbeck  aPlant disease1 aNUNES, M. A.1 aFREITAS-ASTUA, J. de1 aBERGAMINI, M. P.1 aBASTIANEL, M.1 aNOVELLI, V. M.