01852naa a2200229 a 450000100080000000500110000800800410001902400420006010000170010224501450011926000090026452011510027365000150142465000090143965000220144865000100147065000130148070000230149370000170151670000150153377300740154818715652018-05-29       2010    bl uuuu     u00u1 u    #d7 a10.1111/j.1744-7348.2010.00447.x2DOI1 aVERZA, N. C.  aTranscription factor profiling identifies an aleurone-preferred NAC family member involved in maize seed development.h[electronic resource]  c2010  aTranscription factors (TFs) preferentially expressed in developing endosperm were identified by searching a maize transcriptome database. One thousand and two hundred TFs were annotated, 414 of which were shown to express in the seeds. Of these, 113 presented preferential expression in developing endosperm. Among the endosperm-preferred TFs 13% were represented by members of the zinc-finger family, 17% by members of the bZIP family and 11% by members of the NAC family. Among the NAC family TFs, we identified APN-1, whose expression was detected 5 days after pollination (DAP) and peaked at 20 DAP. Analysis of the promoter sequence of gene encoding APN-1 revealed the presence of cis-elements related to endosperm-specificity, abscisic acid (ABA) and gibberellic acid (GA) signalling. Transient expression analysis of a construct containing the ?-glucuronidase gene driven by the Apn-1 gene promoter showed that expression was restricted to the aleurone cell layer. Analysis of mRNA levels in developing maize endosperm treated with hormones revealed that the expression of the gene encoding APN-1 was stimulated by ABA and inhibited by GA.  aEndosperma  aGene  aGenética vegetal  aMilho  aZea mays1 aFIGUEIRA, T. R. S.1 aSOUSA, S. M.1 aARRUDA, P.  tAnnals of Applied Biology, Cambridgegv. 158, n. 1, p. 115-129, 2010.