02428nam a2200241 a 450000100080000000500110000800800410001910000160006024501090007626001440018550001040032952015620043365000250199565000160202065000220203665000190205865000110207765300180208870000170210670000190212370000190214270000250216118705022011-01-20       2010    bl uuuu     u00u1 u    #d1 aKUBO, K. S.  aTransmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites.  aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.c2010  a085 PS1 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010  aFor many years, CiLV-C was classified as an unassigned rhabdovirus. After the complete sequencing of its genome, CiLV-C was proposed as the type member of a new genus of plant viruses, Cilevirus. This virus was believed to have a very narrow host range, but recent studies have shown that several plant species outside the genus Citrus are hosts of CiLV-C. Viral transmission to citrus plants is time consuming and the way plants defend themselves against CiLV-C is still largely unknown, despite recent efforts to evaluate the response of citrus to this pathogen. With the objective to use a model plant to evaluate the response to this disease, we tested whether or not Arabidopsis thaliana could be a host for the virus. We infested the Columbia ecotype with one, two, three, five or ten viruliferous mites for CiLV-C, with six biological repetitions of each treatment. This experiment was evaluated daily in order to verify the development of symptoms. Necrotic localized lesions started to appear in plants eight days after infestation with two mites. By the tenth day, 16% of the plants infested with ten mites, 32% of the plants with five mites and 50% of the plants with three, two and one mites developed necrotic lesions. Samples were processed for transmission electron microcopy (TEM) analyses and RT-PCR with primers designed to amplify a fragment within the movement protein gene of the virus was performed to assure that the lesions were caused by CiLV-C. It was possible to detect the virus in all symptomatic plants, both by RT-PCR and TEM.  aArabidopsis thaliana  aBrevipalpus  aDoença de Planta  aFruta Cítrica  aVírus  aPlant disease1 aARENA, G. D.1 aKITAJIMA, E. W1 aMACHADO, M. A.1 aFREITAS-ASTUA, J. de