02690nam a2200313 a 450000100080000000500110000800800410001910000240006024501990008426001870028352015930047065000230206365000130208665000260209965000230212565000120214865000100216065000200217065000270219065000090221770000160222670000210224270000170226370000200228070000170230070000260231770000170234370000160236018697702017-01-27       2010    bl uuuu     u00u1 u    #d1 aFALLEIROS, A. M. F.  aHistochemical study of the midgut in resistant Anticarsia gemmatalis (Hübner) (Lepidopterabnoctuidae) larvae to nucleopolyhedrovirus AgMNPV, fed with genotypes of soybean resistant to insects.  aIn: LATIN AMERICAN MEETING OF CHEMICAL ECOLOGY, 1., 2010, Colonia de Sacramento. Abstracts? Colonia de Sacramento: Latin American Association of Chemical Ecology, 2010. p. 116.c2010  aThis study aimed verify the chemical composition of the midgut of resistant A. gemmatalis larvae to its nucleopolyhedrovirus, treated with soybean genotypes with different concentrations of rutin and genistin The caterpillars, obtained at the Embrapa/Soja (Londrina-PR-Brazil), were fed with the following genotypes "in natura": BRS257 (control), BR16, Dowling, PI229358, PI227687 and lACIOO. The midguts were collected, fixed and pracessed for detection of acids and neutral polysaccharides (glycogen/PAS), proteins and lipids. The midgut oflarvae fed on genotypes lAC100 and PI227687 showed strongest PASpositivity in columnar cells, as observed in control treatment, indicating the presence of neutral polysaccharides, particularly glycogen; only the genotype PI229358 caused moderate reaction to PAS in the goblet cells. To total proteins technique, the columnar cells showed a weak positive staining with moderate response to treatment with PI227687; the goblet cells were positive to the genotypes PI229358 and lACIOO. There was a positive reaction for lipids in the columnar cells with PI 229358, IAC 100 and PI 227687. No reaction was detected for acid carbohydrates in the epithelial cells of the insects fed with any of the genotypes. We conclude that the rutin and genistin from resistant genotypes alters the chemical composition of the midgut epithelium in resistant A. gemmatalis larvae, changing the synthesis of compounds related to the digestion, being directly implicated in decreasing of the resistance of the insect to the AgMNPV after feeding on the resistant plants.  aBiological control  aSoybeans  aAnticarsia gemmatalis  aControle biologico  aLagarta  aNinfa  aPraga de planta  aPseudoplusia Includens  aSoja1 aCRUZ, N. A.1 aFORGHIERE, L. R.1 aSILVA, M. R.1 aGALLO, A. R. L.1 aSILVA, L. X.1 aHOFFMANN-CAMPO, C. B.1 aMOSCARDI, F.1 aLEVY, S. M.