02653nam a2200229 a 450000100080000000500110000800800410001910000230006024501030008326001690018630000150035549000080037050000180037852019170039665000140231365000110232765300150233865300110235370000170236470000220238170000200240318676782022-10-24       2010    bl uuuu     u00u1 u    #d1 aOLIVEIRA, E. J. de  aMolecular analysis of some varieties, inbred lines and germplasm of papaya.h[electronic resource]  aIn: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. 1 CD-ROM.c2010  ap.757. v.2  vv.2  aPDF. S18.203.  aThe knowledge of genetic relationship in varieties, inbred lines and papaya hybrids is little studied in Brazil. However, this information may improve the effective-ness of breeding programs. Thus, this study was carried out to analyze the genetic divergence among varieties, inbred lines and papaya germplasm belonging to the Active Papaya Germplasm Bank (APGB) at Embrapa Cassava & Tropical Fruits (CNPMF) using AFLP (Amplified Fragment Lenght Polimorfism) markers. We analyzed 32 genotypes and the AFLP reactions were performed with EcoRI/MseI primers. The matrix of genetic distance was obtained using the similarity coef-ficient of simple matching and the clustering was performed using four methods (single linkage, complete linkage, Ward and UPGMA - unweighted pair-method using an arithmetic average). Three hundred eight three polymorphic bands with molecular weights ranging from 100 to 1380 base pairs were obtained using 11 combination of EcoRI / MseI primers. EcoRI-AAC + MseI-CAA, EcoRI-AAC + MseI-CAG and EcoRI-AAC + MseI-CAT combinations showed the highest number of polymorphic bands, 40, 46 and 47, respectively. On average, 34.8 bands were obtained by primer combination. The UPGMA clustering method was the most efficient with cophenetic correlation of 0.74. At a level of 0.20, six clusters were formed. CMF088 and CMF078 accessions showed the highest genetic similarity (0.10). The main papaya varieties commercially used in Brazil like Sunrise, Golden and Calimosa hybrid, as well as four inbred lines and two germplasm accessions are clustered together, although they cannot be grouped in the same branch. The genetic distance between Sunrise and Golden varieties was 0.14. This observation showed that even arising from mutation and selection within the Sunrise variety, the Golden variety stores considerable genetic variability. Additional variability was observed in the inbred lines.  agermplasm  aMamão  aGermplasma  aPapaya1 aCOSTA, J. L.1 aSANTOS, L. F. dos1 aCARVALHO, F. M.