02467naa a2200241 a 450000100080000000500110000800800410001902200140006010000170007424501150009126000090020652017630021565000200197865000210199865000170201965000220203665300230205865300200208170000180210170000200211970000290213977300570216818598592022-08-22       2010    bl uuuu     u00u1 u    #d  a0567-75721 aSOUZA, E. H.  aMicropropagation of Heliconia rostrata and Heliconia bihai from mature zygotic embryos.h[electronic resource]  c2010  aThe difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated charcoal. For the multiplication stage the best medium was the control treatment with no growth regulators and no activated charcoal. A bacterial contamination appeared during subsequence subculture making the micropropagation of these species unfeasible.  aHeliconia bihai  amicropropagation  aFloricultura  aPlanta Ornamental  aHeliconia rostrata  aZygotic embryos1 aSOARES, T. L.1 aSOUZA, F. V. D.1 aSANTOS-SEREJO, J. A. dos  tActa Horticulturae, Leuvengn. 865, p.315-320, 2010.