01560naa a2200277 a 450000100080000000500110000800800410001910000210006024500890008126000090017030000170017949000090019652007720020565000120097765000200098965300290100965300400103865300470107865300280112565300280115365300100118165300120119170000110120370000180121477300500123210798081999-05-14       1997    bl ---       0--  u    #d1 aPOPHAM, H. J. R.  aGenetic improvement of Helicoverpa zea nuclear polyhedrosis virus as a biopesticide.  c1997  ap.83-91 v.10  vv.10  aHelicoverpa zea nuclear polyhedrosis virus, which was previously registered and commercially produced as a pesticide (Elcar), was genetically improved to control the cotton bollworm, H. zea. A significant reduction in the time required for this virus to kill H. zea larvae was achievede by inserting a mite gene encoding a potent insect-selective neurotoxin gene, tox34, into the viral gene encoding ecdysteroid UDP-glucosyltransferase. Under the control of an early viral promoter, expression of tox34 during infection resulted in 50% mortality or paralysis within 40 h after virus treatment. The ability to genetically improve the properties of this virus as a pesticide provides the opportunity to develop a commercially viable product to contro this pest species.  aInsecta  aHelicoverpa Zea  aBaculovirus recombinante  aEcdysteroid UDP-glucosyltransferase  aHelicoverpa zea nuclear polyhedrosis virus  aInsect-selective toxins  aRecombinnat baculovirus  aTox34  aToxinas1 aLI, Y.1 aMILLER, L. K.  tBiological Controlgv.10, n.2, p.83-91, 1997.