02255nam a2200265 a 450000100080000000500110000800800410001910000200006024501170008026000410019730000110023850000180024952015390026765000170180665000170182365000160184065000180185665000180187465000240189265300130191665300190192965300120194865300140196065300150197417908332018-07-18       1986    bl uuuu  m   00u1 u    #d1 aVALLE, E. R. do  aMethods to induce and synchronize ovulation and to enhance corpus luteum function and fertility in beef females.  aUrbana: University of Illinoisc1986  a102 p.  aPh.D. Thesis.  aIn the first section, a competitive inhibition double antibody enzyme immunoassay was developed using a horseradish peroxidase labelled progesterone conjungate and polystyrene tubes. Polystyrene tubes (12 x 75 mm) were incubated overnight at room temperature with 300 ul of sheep anti-rabbit IgG and washed 3 times with distilled water (1 ml) the next day. Standards or extracted samples in a phosphate buffer (100 ul), conjugate  (100 ul) and rabbit anti-progesterone (100 ul) were added in that order to the tubes and incubated on a rotator at room temperature for 3 hours. Tubes were then washed 3 times with distilled water, 400 ul of O-phenylenediamine (16.8 mM) added and incubated in the dark for 30 minutes. After the addition of 400 ul of sulfuric acid (IN) absorbance at 492 nm was determined. The sheep anti-rabbit IgG was generated with Cohn Fraction II rabbit IgG as the immunogen and purified with ammonium sulfate. The rabbit anti-progesterone antibody was developed against 11 alpha-hydroxy-4-pregnene-3,20-dione-11- hemisuccinate: bovine serum albumin. The cross reactivity was greater than 2% only for 11 alpha-hydroxyprogesterone (22.0%) and 5 alpha-pregnan-3,20-dione (4.0%). The sensitivity of the assay was 5 pg (P&lt.05) and 84% of the progesterone spiked to samples was recovered which was similar to recovery of tritiated progesterone spiked samples (83%). When volumes of 50, 100, 150 and 200 ul of plasma were assayed, progesterone values were 3.63, 3.61, 3.56 and 3.61 (r = .99; P&lt.01). Intraassay and...  ainsemination  areproduction  aFertilidade  aGado de Corte  aInseminação  aReprodução Animal  aBeef cow  aCalf fertility  aNovilha  aPos parto  aPostpartum