01412naa a2200253 a 450000100080000000500110000800800410001910000170006024501060007726000090018352007460019265000160093865000100095465000100096465000170097465300160099165300100100765300110101765300150102870000170104370000200106070000190108077300590109917858312017-04-06       1990    bl ---       0--  u    #d1 aSAFIR, G. R.  aImprovement and synchronization of va mycorrhiza fungal spore germination by short-term cold storage.  c1990  aVesicular-arbuscular (VA) mycorrhizal fungi are ecologically obligate biotrophs that have not been cultivated in defined media in the absence of living roots (Siqueira et al., 1985). However, asexual spores, can be obtained quite abundantly from pot culture with nurse plants, that have been inoculated with the desired species (ferguson and Woodhead, 1982). Freshly-collected resting spores may be readily used as plant inoculum or as starding material for nutritional studies and attempts of axenic culture. Never-theless, these spores may fail to germinate or exhibit slow and erratic germination which may continue over weeks and even months (Tommerupp, 1983) depending upon a number of nutritional, biological and environmental factors.  agermination  aCampo  aFungo  aGerminação  aCurto tempo  aField  aFungus  aShort time1 aCOLEY, S. C.1 aSIQUEIRA, J. O.1 aCARLSON, P. S.  tSoil Biology Biochemistrygv.22, n.1, p.109-111, 1990.