02144nam a2200397 a 450000100080000000500110000800800410001910000160006024501120007626000560018830000090024450000190025352012030027265000130147565000120148865000120150065000130151265000090152565000160153465000120155065000120156265000090157465000130158365000110159665000110160765300120161865300120163065300120164265300220165465300110167665300100168765300190169765300090171665300100172565300110173517522812013-06-20       1979    bl uuuu  m   00u1 u    #d1 aHAAS, J. C.  aEndogenous levels of sugars and growth of tomato roots and callus cultured on sucrose, glucose or fructose.  aNew Brunswick: State University of New Jerseyc1979  a51p.  aTese Mestrado.  aThe technique of plant tissue culture, which uses defined media and controlled environments in the culture of excised plant parts, has been developed since the thirties. With this type of technique many plant cultures can be propagated vegetatively and clones can be established. Materials obtained by clonal culture are useful in studies which do not require genetic variations. As the composition of the medium and the environment in which the cultures are grown can be controlled, the cultures are useful in studying the nutritional requirements, morphogenesis, nutrient absorption, senescence, and other physiological activities of plants. The establishment of excised root cultures from radicle tips was first attempted by Kotte (1922 a,b) and Robbins (1922 a, b).The tips placed in a culture media containing glucose, mineral salts and a complex organic supplement were able to grow into roots of normal morphology. Robbins, working with maize roots, attempted to effect their subculture by excising apical meristems to new flasks of culture medium. Such root tips were capable of further growth, but at each subculture the growth rate declined and ultimately all the cultures died out.   ...  afructose  aglucose  asucrose  aAçúcar  aCalo  aCrescimento  aFrutose  aGlicose  aRaiz  aSacarose  aTecido  aTomate  aCultivo  aCultura  aCulture  aEndogenous levels  aGrowth  aNivel  aNivel endogeno  aRoot  aSugar  aTomato