02177naa a2200277 a 450000100080000000500110000800800410001902400570006010000160011724500970013326000090023052013360023965000200157565000200159565300130161565300180162870000170164670000170166370000240168070000270170470000200173170000170175170000220176870000250179077300840181516973682024-02-05       2009    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1590/S1415-475720090050000742DOI1 aFONSECA, I.  aExpression profile of genes associated with mastitis in dairy cattle.h[electronic resource]  c2009  aIn order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p &lt 0.05). It was also higher in BW Holstein animals with clinical mastitis (p &lt 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 &lt p &lt 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-γ genes (p &lt 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.  agene expression  aimmune response  aCytokine  aReal-time PCR1 aSILVA, P. V.1 aLANGE, C. C.1 aGUIMARAES, M. F. M.1 aWELLER, M. M. D. C. A.1 aSOUSA, K. R. S.1 aLOPES, P. S.1 aGUIMARÃES, J. D.1 aGUIMARÃES, S. E. F.  tGenetics and Molecular Biology, Ribeirão Pretogv. 32, n. 4, p. 776-781, 2009.