01861naa a2200277 a 450000100080000000500110000800800410001902400530006010000230011324501250013626000090026152009780027065000340124865300220128265300100130465300100131465300240132470000220134870000210137070000190139170000170141070000190142770000200144670000200146677300970148616970512024-02-16       2009    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.cimid.2008.07.0062DOI1 aALVES, C. C. de S.  aCellular and humoral immune responses during intrathoracic paracoccidioidomycosis in BALB/c mice.h[electronic resource]  c2009  aParacoccidioidomycosis is a chronic infection that primarily affects the lungs. Here we investigated cellular and humoral immune responses after intrathoracic Paracoccidioidesbrasiliensis infection in BALB/c mice. P. brasiliensis-colony-forming units (CFUs), fungal DNA and granulomas in lungs increased progressively, peaking at day 90 postinfection (p.i.). IFN-γ production was highest on day 15 p.i., declining thereafter. The kinetics of the NO production was similar to that described for IFN-γ. In contrast, IL-10 increased from day 45 p.i. reaching a peak at day 90. Levels of serum IgG1 were higher than IgG2a between days 30 and 90 p.i. 30% of mice died by day 90 p.i. These data indicate that infection with P. brasiliensis by the intrathoracic route shows high IFN-γ and NO production at day 15 p.i., unable to control multiplication of fungi, which appears to be associated with a progressive increase in IL-10 and in the number and complexity of granulomas.  aParacoccidioides brasiliensis  aAntibody response  aIFN-y  aIL-10  aIntrathoracic route1 aAZEVEDO, A. L. S.1 aRODRIGUES, M. F.1 aMACHADO, R. P.1 aSOUZA, M. A.1 aMACHADO, M. A.1 aTEIXEIRA, H. C.1 aFERREIRA, A. P.  tComparative Immunology, Microbiology and Infectious Discasesgv. 32, n. 6, p. 513-525, 2009.