01687naa a2200301 a 450000100080000000500110000800800410001902400350006010000170009524500870011226000090019952008170020865000260102565000250105165000230107665000350109965000130113465300160114765300200116370000200118370000140120370000200121770000200123770000190125770000180127670000200129477300710131416617992010-04-27       2009    bl ---       0--  u    #d7 a10.1007/s12686-009-9111-y2DOI1 aJUNGMANN, L.  aDevelopment of microsatellite markers for Brachiaria humidicola (Rendle) Schweick.  c2009  aWe describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X–9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.  aBrachiaria Humidicola  aGramínea Forrageira  aMarcador Molecular  aMelhoramento Genético Vegetal  aPastagem  aBraquiária  aMicrossatélite1 aVIGNA, B. B. Z.1 aPAIVA, J.1 aSOUSA, A. C. B.1 aVALLE, C. B. do1 aLABORDA, P. R.1 aZUCCHI, M. I.1 aSOUZA, A. P. de  tConservation Genetics Resourcesgv. 1, n.1, p. 475-479, Dec. 2009.