01983nam a2200241 a 450000100080000000500110000800800410001910000230006024501290008326001310021252012230034365000110156665000220157765000100159965000110160965000140162065000190163470000180165370000220167170000150169370000140170870000190172216555432023-07-07       2008    bl uuuu     u00u1 u    #d1 aDÍAZ-TRUJILLO, C.  aA reliable routine assay for black leaf streak disease of bananas caused by Mycosphaerella fijiensis.h[electronic resource]  aIn: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburghc2008  aMycosphaerella fijiensis (Paracercospora fijiensis), an ascomycete fungus, is the causal agent of Black Sigatoka (also know as black leaf streak disease), the most devastating foliar disease of banana and plantain (Musa spp.). This pathogen is a global threat to banana plantations, demanding a major input of fungicides for its control. In some banana production areas in South and Central America more than 50 sprays are required annually to control this disease. The genome sequence of M. fijiensis CIRAD86 was recently made publicly accessible (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html). The availability of this genome database is essential to increase our understanding of this host-pathogen interaction. The molecular aspects of the interaction are largely unknown. In order to study these we developed a reliable infection protocol for routine phenotyping assays and specific melocular/expression studies. During the biotrophic stage, which can last for over three weeks, M. fijiensis does not trigger macroscopic symptoms. These only appear during the necrotrophic phase, which onset varies with pathogen virulence, nutrients availability, environmental conditions and susceptibility of the cultivar.  aBanana  aDoença de Planta  aFungo  aGenoma  aPatógeno  aSigatoka Negra1 aFORTES, C. F.1 aCAPDEVILLE, G. de1 aJALINK, H.1 aSOUZA, M.1 aKEMA, G. H. J.