02228nam a2200289 a 450000100080000000500110000800800410001910000170006024501910007726001310026850000110039952012810041065000110169165000220170265000100172465000220173465000190175670000260177570000150180170000190181670000160183570000200185170000190187170000150189070000140190570000190191916555422023-07-07       2008    bl uuuu     u00u1 u    #d1 aFERREIRA, C.  aDevelopment of cost effective tools for assessing genetic diversity of Mycosphaerella fijiensis, the causal agent of black-Sigatoka disease in bananas (Musa spp.).h[electronic resource]  aIn: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburghc2008  aPR9.29  aMycosphaerella fijiensis, the causal agent of banana leaf streak disease, commonly know as Black Sigatoka disease, is one of the most devastating plant pathogens. M. fijiensis is a relatively new pathogen and still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://www.genome.jgi-psf.org/Mycfi1/Mycfi1.home.html) we identified variable number of tandem repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono - di - or tri - nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on progeny isolates and field isolates. The markers allowed robust scoring on agarose gels and proved useful for variability and population genetic studies using basic molecular instrumentation. We envisage that this set will allow more detailed analysis of the large scale migration pattern of this important pathogen and the small scale effect of fungicide tratments on the population diversity in banana plantations.  aBanana  aDoença de Planta  aFungo  aGenética Vegetal  aSigatoka Negra1 aVan der LEE, T. A. J.1 aQUIROS, O.1 aHERRERT, B. L.1 aCARLIER, J.1 aTRUJILLO, C. D.1 aGOODWIN, S. B.1 aGUZMAN, M.1 aSOUZA, M.1 aKEMA, G. H. J.