01863naa a2200217 a 450000100080000000500110000800800410001910000230006024501300008326000090021352011280022265000220135065000110137265300110138370000180139470000170141270000150142970000200144470000190146477301620148316553602009-01-15       2008    bl uuuu     u00u1 u    #d1 aFREITAS-ÁSTUA, J.  aEvidences to support that Citrus leprosis virus and its mite vector interact in a circulative - but not propagative - manner.  c2008  aCitrus leprosis is considered the most important viral disease for the Brazilian citrus industry mainly because of the high costs involved in controlling its mite vector Brevipalpus phoenicis (Acari: Tenuipalpidae). Until recently restricted to few countries in South America, leprosis is now spread throughout several countries in the Continent, and has reached Mexico in North America. The disease is unusual, since Citrus leprosis virus (CiLV) induces only localized lesions in its susceptible hosts, such as Citrus sinensis. This characteristic increases the importance of the vector in the epidemiology of the disease, since the CiLV dissemination in the field is strictly dependent on the mite. It has been reported that the CiLV-Brevipalpus interaction is of propagative manner, but biological data obtained by our group did not support such hypothesis. In order to determine the type of virus-vector interaction for the leprosis pathosystem, we developed a RT-qPCR-based test to detect and quantify CiLV in viruliferous mite vectors. Our data indicated that CiLV circulates - but does not propagate - in its vector.  aDoença de Planta  aVírus  aCitros1 aBASTIANEL, M.1 aNICOLINI, F.1 aSCHONS, J.1 aKITAJIMA, E. W.1 aMACHADO, M. A.  tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan, China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 237-238. P349