01884naa a2200265 a 450000100080000000500110000800800410001902000180006010000160007824501210009426000090021530000140022450002520023852009030049065000130139365000110140665300240141765300150144165300150145665300150147165300260148670000180151270000220153077300660155216458452000-05-16       2000    bl uuuu     u00u1 u    #d  a85-87697-05-61 aALVAREZ, E.  aDNA polymorphism and virulence variation of a Phytophthora population isolated from cassava Manihot esculenta Crantz  c2000  ap.279-287  aINTERNATIONAL SCIENTIFIC MEETING ON CASSAVA BIOTECHNOLOGY, 1998. Salvador-Bahia. Proceedings... Brasilia, DF: Embrapa Recursos Geneticos e Biotecnologia/CBN, 2000. 625p. Editado por Luiz J. C. B. Carvalho, Ann Marie Thro, Alberto Duarte Vilarinhos  aCassava (Manihot esculenta Crantz) has great social importance as it is the principal carbohydrate source in developing countries. A major factor in cassava production in Latin America is root rot a destructive and widespread disease. The understanding of the root rot causing pathogens and the virulence variation in Phytophthora is essential to effectively for root rot resistance. One hundred twenty two isolates of Phytophthora were obtained by a direct plating method and by a bating technique using in vitro fragments of cassava propagated plantlets as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of diffferent Phytophthora species were also included in the molecular analysis. In addition theisolates were identified by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization using a Phytophthora specific probe.  aMandioca  aVírus  aDiseease resistance  aMorfologia  aMorphology  aProduction  aResistencia a doencas1 aCHACON, M. I.1 aCUSGUEN, S. N. J.  tBrasilia: Embrapa Recursos Genetico e Biotecnologia/CBN, 2000