02137naa a2200385 a 450000100080000000500110000800800410001910000140006024500980007426000090017230000100018150001230019152010880031465000110140265000130141365000140142665000200144065000120146065000170147265000240148965000230151365300110153665300140154765300130156165300140157465300080158865300120159665300100160865300130161865300180163170000190164970000290166870000200169777300340171716447901997-05-22       1996    bl uuuu     u00u1 u    #d1 aHE, C. X.  aEric-pcr for early detection of bacterium (Xylella fastidiosa) of citrus variegated chlorosis  c1996  ap.447  aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR.  aCitrus variegated chlorosis (CVC) caused by a Gram-negative bacterium Xylella fastidiosa. Since it is graft-and vector-transmissible, the disease threatens to spread into all of South, Central and North America. It is in need to develop and early diagnosis of the disease. Enterobacterial repetitive intergenic conssensus (ERIC) sequences in conjunction with the polymerase chain reaction techniques (PCR) was developed and used to detect the pathogen of CVC. It was proved that the ERIC-PCR technique is a powerful tool for early detection of the bacteria in the assymptomatic plants affected by CVC. The bacteria of CVC Xylella fastidiosa from the assymptomatic seedlings with artificial inoculation of trifoliate orange (P. trifoliata (L.) Raf.), Troyer citrange (C. sinensis x P. trifoliata), Swingle citrumelo (C. paradisi x P. trifoliata), and assymptomatic citrus nursery trees and plants in orchards were detected by the ERIC-PCR technique. Some differences of the bacterium DNA patterns were observed, suggesting that Xylella fastidiosa of CVC should have various in Brazil.  aCitrus  aPoncirus  aBactéria  aCitrus Sinensis  aDoença  aLaranja Doce  aPoncirus Trifoliata  aXylella Fastidiosa  aCitros  aCitrus sp  aCongress  aCongresso  aCVC  aDisease  aMudas  aSeedling  aSweet oranges1 aCIAPINA, L. P.1 aDONADIO, L. C. LI, W. B.1 aLEMOS, E. G. M.  tCuritiba, PR: IAPAR/SBF, 1996