02111naa a2200241 a 450000100080000000500110000800800410001910000160006024501460007626000090022230000140023150001200024552013070036565000110167265000120168365000100169570000150170570000140172070000210173470000150175570000160177077300830178616442851993-10-15       1991    bl uuuu     u00u1 u    #d1 aGARNIER, M.  aStudy of the greening organism (GO) with monoclonal antibodiesbSerological identification, morphology, seroptypes and purification of the GO  c1991  ap.428-435  aConference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Procedings...  aA monoclonal antibody (MA) previously developed to a strain of the greening organism (GO) from Poona, India, was used to purify the homologous organism from infected periwinkles. Immunofluorescence and immunogold labeling were used to identify the GO before and after purification . Isolated cells of the GO could be observed for the first time in the electrom microscope. Both filamentous forms and roud forms were seen. The filamentous forms were 1 to 4 um. the diameter of their cross section varied between 0.1 and 0.3 um. The round forms had a diameter of 0.2 to 1 um. We have also prepared several MA against a strain of the GO from Fujian province, China. These MA were able to detect the homologous GO strain but not the Indian strain of the GO. The MA against the Indian strain detected the homologous Indian strain as well as strains from Assam, Delhi and Shillong (India), Bali and Java (Indonesia) and Lipa city (Philipines); they did not detect strains from China, Thailand and Malaysia; several strains from India, Indonesia and the Philipines. These results demonstrate for the first time that different stereotypes of the GO exist, even within the same geographical area. Preparation of polyclonal antibodies and DNA probes less specific than MA is in progress to detect all GO strains.  aCitrus  aDoença  aElisa1 aGAO, S. J.1 aHE, Y. L.1 aVILLECHANOUX, S.1 aGANDAR, J.1 aBOVE, J. M.  tRiverside, California: International Organization of Citrus Virologists, 1991.