01556naa a2200205 a 450000100080000000500110000800800410001910000130006024501120007326000090018530000120019450001200020652008770032665000140120365000100121765300120122765300170123970000110125677300830126716442171993-09-09       1991    bl uuuu     u00u1 u    #d1 aTSAI, M.  aDevelopment and characterization of monoclonal antibodies to citrus tristeza virus (CTV) strains in Taiwan.  c1991  ap.46-50  aConference of the International Organization of Citrus Virologists, 11., 1991, Riverside California, Proceedings...  aTwelve stable hybridoma cell lines secreting CTV-specific antibodis were obtained against purified sources of SY-M, a mild seedling-yellows strain of CTV, and CTV-D, a non-seedling-yellows dwarfing strain. These monoclonal antibodies (MAB) included IgG 1, IgG 2a, and IgM subtypes. Immunuglobulin titers up to 4,096 X and 83,886,080 X were obtained in culture supernatants and ascites fluid respectively. To MABs were found which reacted differently to mild and severe CIV strains in double antibody sandwich-indirect ELISA. Thirtheen domestic and foreing isolates of CTV showed six distinct reaction patterns when tested with the two MABs from Taiwan plus MABMCA13 from Florida and MAB 3DF1 from Spain The MAB 4H6-1 is distinct from previously described MAB. The MAB 10E3-4 reacts similary to MCA13, but shows at least some quantitative differences with several isolates.  aserotypes  aElisa  aPummelo  aStem pitting1 aSU, H.  tRiverside, California: International Organization of Citrus Virologists, 1991.