01636naa a2200265 a 450000100080000000500110000800800410001902200140006010000180007424501140009226000090020652008640021565000210107965000300110065000190113065300190114965300080116865300200117665300240119670000210122070000170124170000200125870000180127877300740129616328311993-10-06 1993 bl uuuu u00u1 u #d a0014-23361 aJARRET, R. L. aDetecting genetic diversity in diploid bananas using PCR and primers from a highly respectitive DNA sequence. c1993 aThe polymerase chain reaction (PCR) was used to detect polymorphisms among 29 diploid clones of Musa acuminata Colla. from Papua New Guinea. Primer sequences were derived from a 520 bp highly respectitive DNA sequence isolated from M.acuminata ssp. malaccensis. Primers, used individually, detected a total of 48 polymorphisms that were scored as unit characters and used to generate a Jaccard's similarity index. Principal coordinate analysis (PCO) was used to cluster clones and the unweighted paired-group method of analysis (UPGMA) was used to compute genetic distance among the materials examined. The abundance of diversity within the PNG diploids examined reflects the exterme genetic variability within the M.acuminata gene pool. PCR,utilizing primes from a highly respetitive sequence, is a rapid means of detecting genetic diversity in M. acuminata. aPapua New Guinea apolymerase chain reaction aMusa Acuminata aDiploid banana aPCR aPlant germplasm aRepetitive sequence1 aVUYLSTEKE, D. R.1 aGAWEL, N. J.1 aPIMENTEL, R. B.1 aDUNBAR, L. J. tEuphytica International Journal of Plant Breedinggv.68, n.1-2, 1993.