01915naa a2200253 a 450000100080000000500110000800800410001910000270006024501130008726000090020050000270020952011360023665000120137265000210138465300330140565300080143865300110144665300080145765300410146570000230150670000200152970000240154977300880157316299392015-03-16       2008    bl uuuu     u00u1 u    #d1 aSIMÕES-ARAÚJO, J. L.  aVuNIP1 (NOD26-like) and VuHSP17.7 gene expression are regulated in response to heat stress in cowpea nodule.  c2008  aParceria: UFRJ; UFRGS.  aIn tropical areas, high soil temperatures frequently limit biological nitrogen fixation (BNF) activity in a number of legumes species. In contrast with other legumes growing under BNF. cowpea (Vigna unguiculata) nodules are very resistant to high-temperature stress. However, the molecular basis of nodule heat tolerance remains unclear. In order to identify genes involved in the heat stress response in cowpea nodules a eDNA library was screened with cDNA-AFLP derived probes. Two full-length cDNAs corresponding to a small heat shock protein (VuHSP 17.7) and to a Nodulin 26 (VuNIP I) were isolated and characterized. VuHSPI7.7 encodes a sHSP family class I protein, and VuNIPI corresponds to a NOD26-like protein, member of NIP subfamily of plant membrane intrinsic protein (MIP). VuHSP 17.7 was highly induced by high-temperature stress in nodule, leaves, flower. and bud flower, and VuNIPI was repressed in nodule after heat stress. Moreover, the spatial expression pattern of VuNIPI and VuHSPI7.7 differs significantly indicating that distinct signaling pathways under heat stress may regulate the expression of these genes.  acowpeas  aFeijão de Corda  aBiological nitrogen fixation  aBNF  aCowpea  aFBN  aFixação bioloógica de nitrogênio1 aALVES-FERREIRA, M.1 aRUMJANEK, N. G.1 aMARGIS-PINHEIRO, M.  tEnvironmental and Experimental Botany, Oxfordgv. 63, n. 1-3, p. 256-265, may 2008.