01710naa a2200205 a 450000100080000000500110000800800410001910000120006024501120007226000090018452011450019365000110133865000200134970000110136970000160138070000120139670000120140870000110142077300730143115985692019-07-08       2006    bl ---       0--  u    #d1 aXIE, R.  aIdentification of genes induced during Medicago sativa nodule development by using the cDNA-AFLP technique.  c2006  aA variety of genes involved in nodule development and function were induced during sym-biotic root nodule development in alfalfa. We identi-fied those genes by comparing the eDNA-amplified fragment length polymorphism (cDNA-AFLP) pat-terns of infected nodules and uninfected roots. Sev-enty-one transcript-derived fragments (TDFs) were isolated from about 3000 TDFs, which are enhanced or specifically expressed in nodule. The differential expression patterns of 61 genes were confirmed by reverse Northern hybridization or Northern hybridiza-tion analyses. Among these, 31 exhibited significant similarities to characterized database entries. These results suggest that the genes corresponding to the 31 TDFs may play important roles in signal transduc-tion, gene expression and regulation, substance transportation or auto-regulation of nodulation during nodule development. The Northern hybridization analysis also indicates that the gene corresponding to TDF RX89 was not expressed in the nodule elic-ited by bacA mutant, presumably it is specially in-duced at the stage of nodule development when bacteroids begin to be differentiated.  aAlfafa  aMedicago Sativa1 aHE, Z.1 aFANGHUA, L.1 aZOU, H.1 aZHU, J.1 aYU, G.  tChinese Science Bulletin, Beijinggv. 51, n. 17, p. 2087-2094, 2006.