01747naa a2200277 a 450000100080000000500110000800800410001902400350006010000210009524500990011626000090021552009770022465000160120165000180121765000130123565000120124865000160126065000090127665300270128565300120131270000190132470000200134370000190136370000210138277300660140315797712023-01-24 2009 bl uuuu u00u1 u #d7 a10.1007/s00705-009-0463-02DOI1 aFERNANDES, F. R. aThree distinct begomoviruses associated with soybean in central Brazil.h[electronic resource] c2009 aWe report the complete nucleotide sequences of geminiviruses of the genus Begomovirus infecting soybean (Glycine max) in central Brazil. Samples obtained from soybean plants collected at Santo Antonio de Goiás, Goiás State, showing typical symptoms of viral infection, were analyzed. Infection was confirmed by PCR-based amplification of a DNA-A fragment with universal begomovirus primers. Total DNA from infected plants was then subjected to rolling-circle amplification (RCA), and 2.6-kb molecules were cloned into plasmid vectors. Sequencing of the three DNA-A and two DNA-B clones thus obtained confirmed infection by three distinct begomoviruses: bean golden mosaic virus, Sida micrantha mosaic virus and okra mottle virus, the last of which was reported recently to be a novel virus infecting okra plants in Brazil. Begomovirus infection of soybean plants has been reported sporadically in Brazil and has generally not been considered to be of economic relevance. aBegomovirus aGeminiviridae aSoybeans aCerrado aGlycine max aSoja aRegião Brasil Central aSoybean1 aCRUZ, A. R. R.1 aFARIA, J. C. de1 aZERBINI, F. M.1 aARAGAO, F. J. L. tArchives of Virologygv. 154, n. 9, p. 1567-1570, Sept. 2009.