01696naa a2200301 a 450000100080000000500110000800800410001910000260006024501020008626000090018830000150019752008230021265000120103565000120104765000130105965000110107265000100108365000170109365000110111065000130112165000220113465000140115665300170117070000230118770000200121070000200123077301440125015564141996-09-03       1993    bl uuuu     u00u1 u    #d1 aCARVALHO, L. J. C. B.  aStudies on proteins and enzymes related to tuberization and starch biosynthesis in cassava roots.  c1993  ap.234-238.  aA method has been developed to isolate intact cassava root amyloplast through protoplast preparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous ficoll density agradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch symthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tube-rization project that is undergoing in CENARGEN.  acassava  aenzymes  aproteins  astarch  aAmido  aBiossíntese  aEnzima  aMandioca  aManihot Esculenta  aProteína  aByosynthesis1 aCASCARDO, J. C. M.1 aFERREIRA, M. A.1 aLOUREIRO, M. E.  tIn: INTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHNOLOGY NETWORK, 1., 1992, Cartagena de Indias, Colombia. Proceedings. Cali: CIAT, 1993.