03757naa a2200589 a 450000100080000000500110000800800410001902400430006010000200010324501610012326000090028452021520029365000250244565000180247065000180248865000120250665000140251865000100253265000200254265000170256265000210257965000120260065000120261265000220262465000170264665000150266365000170267865000140269565000160270965000180272565000120274365000120275565000170276765300150278465300170279965300230281665300250283965300110286465300160287565300100289165300250290165300150292665300160294170000210295770000200297870000190299870000200301770000230303770000190306070000220307977300660310115306662024-04-10       2004    bl uuuu     u00u1 u    #d7 a10.1016/j.anireprosci.2003.09.0062DOI1 aSILVA, J. R. V.  aSurvival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water.h[electronic resource]  c2004  aAbstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration.  aBovine serum albumin  aCell division  aCoconut water  aFemales  aGlutamine  aGoats  aGranulosa cells  aHypoxanthine  aIn vitro culture  aInsulin  aOocytes  aOvarian follicles  aPharmacology  aPhysiology  aReproduction  aSolutions  aTransferrin  aÁgua de Coco  aCaprino  aOvário  aReprodução  aActivation  aDrug effects  aFolículo ovariano  aFolículo primordial  aGrowth  aMaintenance  aOvary  aPrimordial follicles  aSuplemento  aSupplements1 aHURK, R. van den1 aCOSTA, S. H. F.1 aANDRADE, E. R.1 aNUNES, A. P. A.1 aFERREIRA, F. V. A.1 aLOBO, R. N. B.1 aFIGUEIREDO, J. R.  tAnimal Reproduction Sciencegv. 81, n. 3/4, p. 273-286, 2004.