03256naa a2200349 a 450000100080000000500110000800800410001902400490006010000180010924501900012726000090031752021290032665000150245565000200247065000220249065000180251265000100253065000250254065000100256565000170257565000260259265000150261865000240263365000250265765300280268265300310271070000160274170000210275770000180277870000330279677300770282915303542023-08-28       1996    bl uuuu     u00u1 u    #d7 aDOI: 10.1111/j.1439-0450.1996.tb00335.x2DOI1 aRODRIGUEZ, F.  aAn immunohistochemical method of detecting mycoplasma species antigens by use of monoclonal antibodies on paraffin sections of pneumonic bovine and caprine lungs.h[electronic resource]  c1996  aLung sarnples from pneumonic lesions in cattle and goats, natura1ly or experimentally infected with strains of the Mycoplasma mycoides cluster, were fixed in formalin and embedded in paraffin. An immunohistochemical technique using monoclonal or polyclonal antibodies was performed on tissue sections in order to detect Mycoplasma antigens. Four monoclonal antibodies (MAbs), one (2A3) raised against M. mycoides ssp. mycoides small colony (SC) and large colony (LC), twO (ID3 and SES) against M. mycoides ssp. capri, and one (5A10) against M. bovis, were used. A range of polyclonal antibodies, raised to the individual subspecies of the M. mycoides cluster, and one to Pas/eure/la haemo!ytica, was also used. The MAb 2A3 showed positive immunostaining in lung sections from cattle and goats natura1ly and experimentally infected with M. mycoides ssp. mycoides SC and LC, but not with pneumonic lesions of cattle and goats due to other members of the M. mycoidts cluster, M. bovis or Pasteure/la spp. The MAb ID3 showed immunostaining in lung sections from goats naturally and experimentally infected with M. mycoides ssp. capri, but again not with pneumonic lesions caused by other members of the M. mycoidts cluster, M. bovis or Pasteurella spp. The MAb SES immunoreacted in sections from pneumonic lesions from ail animais infected with one of the three M.mycoides cluster subspecies used in the study, but not with M bovis or Pasteurella infected tissue. lmmunoreaction was mainly found in the cell debris around necrotic areas, as well as in macrophages, neutrophils and epithelial cells. The localization of antigens of the M. mycoides cluster using polyclonal antisera followed basically the same pattern as mat obtained with the monoclonals. However, a wide cross reactivity was found between different antisera and relatively high background immunostaining was also seen, especially in necrotic areas. The results suggest that immunohistochemical methods using monoclonal antibodies are useful tools for the diagnosis and study of the pathogenesis of pneumonia caused by the Mycoplasmas of the M. mycoides cluster.  aAntibodies  aCattle diseases  aDisease diagnosis  aGoat diseases  aGoats  aIsolation techniques  aLungs  aMicrobiology  aMonoclonal antibodies  aMycoplasma  aMycoplasma mycoides  aPurification methods  aImmunoenzyme Techniques  aPleuropneumonia Contagious1 aKENNEDY, S.1 aBRYSON, T. D. G.1 aFERNANDEZ, A.1 aRODRIGUEZ, J. L. BALL, H. J.  tJournal Veterinary Medicinegv. 43, n. 1/10, p. 429-438, Jan./Fev. 1996.