03210naa a2200205 a 450000100080000000500110000800800410001910000220006024501210008226000090020352026020021265000100281465000130282465000120283765300290284970000150287870000150289370000210290877300750292915287922002-10-31       2000    bl uuuu     u00u1 u    #d1 aDRIANCOURT, M. A.  aControl of ovarian follicular growth and maturation by the corpus luteum and the placenta during pregnancy in sheep.  c2000  aOvarian follicular growth and maturation and its control throughout pregnancy have not been described fully in sheep. Experiment 1 characterized the size and maturation (steroid production in vitro and aromatase activity) of ovarian follicles obtained at days 20, 50, 80 and 110 of pregnancy compared with those obtained at day 12 of the oestrous cycle. There was no difference in the number of small follicles « 3 mm in diameter) between cyclic and pregnant ewes, regardless of the stage of pregnancy. There was a marked reduction (P &lt 0.01) in the number of medium follicles (3-5 mm) starting at day 80 of pregnancy. Large follicles (&gt 5 mm) were not detected at day 110 of pregnancy. In vitro testosterone output by follicles was constant throughout pregnancy. Oestradiol t output remained steady until day 80, but decreased markedly at day 110 of pregnancy. This decrease was associated with a reduction in aromatase activity in follicles obtained r: at this stage. Experiment 2 examined the effect of administration of high concentrations of progesterone between day 100 and day 120 after mating on resumption of follicular r growth in ewes that underwent Caesarean section at day 99 of pregnancy. In ewes that underwent Caesarean section, progesterone supplementation was successful in mimicking the profile found in pregnant ewes, but did not prevent re-initiation of follicular growth, as demonstrated by the presence of large follicles (&gt 5 mm) at day 120 after mating. Experiment 3 examined the effects of PGF 2alpha-induced regression of the corpus luteum of day 100 of pregnancy on resumption of follicular growth. High concentrations of PGF2alpha (0.28 mg kg-l body weight) administrated at day 100 of pregnancy were required to initiate regression of the corpus luteum. At day 120 after mating, the mean (+- SEM) diameter of the largest follicle in PGF2alpha-treated ewes (3.40 :t 0.47 mm) was significantly greater (P &lt 0.05) than that in control pregnant ewes (2.52 +- 0.34 mm). Experiment 4 examined the effect of removal of the fetus and of the corpus luteum at day 100 of pregnancy on resumption of ovulation. Removal of the corpus luteum by PGF 2alpha treatment at the time of removal of the fetus resulted in earlier occurrence of short luteal phases (27.8 versus 40.6 days, PGF2alpha-treated versus non-treated) but did not alter the timing of the first normal luteal phases (41 days). In conclusion, the results from these experiments indicate that placental compounds play a major role in inhibiting follicular growth and maturation during late pregnancy in sheep.  aOvino  aPlacenta  aPrenhez  aHormônio de crescimento1 aFÉVRE, J.1 aMARTAL, J.1 aAL-GUBORY, K. H.  tJournal of Reproduction and Fertilitygv. 120, n. 1, p. 151-158, 2000.