02248naa a2200349 a 450000100080000000500110000800800410001902400540006010000170011424501620013126000090029352011220030265000250142465000230144965000170147265000200148965000230150965000380153265000190157065000150158965000210160465000390162565000190166465000390168365000120172265000240173465000100175870000160176870000170178470000170180177300800181815233392023-06-23       1991    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0165-2427(91)90122-S2DOI1 aELLIS, J. A.  aAntigen specificity and activity of ovine antibodies induced by immunization with Corybebacterium Pseudotuberculosis culture filtrate.h[electronic resource]  c1991  aAbstract: Sheep were immunized three times with a vaccine composed of filtrate from a 36 h culture of Corynebacterium pseudotuberculosis and a block polymer adjuvant. Immunization resulted in the development of exotoxin-neutralizing antibody. This corresponded to the recognition of a 31.6 kDa protein on sequential immunoblots of ammonium sulfate-precipitated filtrate. In addition, sera from vaccinated sheep recognized at least eight bacterial cellular antigens on immunoblots of ether-extracted C. pseudotuberculosis, including bands of 12, 25.1, 31.6, 36.3, 39.8, 63.1, 70, 75 or 79.4 kDa. Sera from these sheep altered the colony growth characteristics of C. pseudotuberculosis in vitro. These results indicate that immunization with soluble C. pseudotuberculosis antigen preparations that have been used in toxoid vaccines induces antibody responses to numerous cellular antigens in addition to exotoxin and suggest that serologically mediated antibacterial effects could be an important component in the protection from disease that has been reported following immunization with C. pseudotuberculosis toxoids.  aBacterial antibodies  aBacterial vaccines  aBiosynthesis  aDisease control  aDisease prevention  aEnzyme-linked immunosorbent assay  aImmunoblotting  aImmunology  aMolecular weight  aPolyacrylamide gel electrophoresis  aSheep diseases  aCorynebacterium Pseudotuberculosis  aDoença  aLinfadenite Caseosa  aOvino1 aHAWK, D. A.1 aMILLS, K. W.1 aPRATT, D. L.  tVeterinary Immunology and Immunopathologygv. 28, n. 3/4, p. 303-316, 1991.