02169naa a2200337 a 450000100080000000500110000800800410001902200140006010000230007424501380009726000090023552011980024465000170144265000120145965000130147165000220148465000110150665000350151765000220155265000200157465300190159465300210161365300140163465300120164865300190166065300210167965300240170070000180172470000230174277300660176514921332021-06-28 1995 bl uuuu u00u1 u #d a0100-84551 aOLIVEIRA, M. N. de aMechanisms of 2n potato pollen formation in dihaploid Solanum tuberosum L. x S. chacoense Bitt. hybrid clones.h[electronic resource] c1995 aThe backcrosses of dihaploid Solanum tuberosum with wild species hybrids generating tetraploids progenies require the formation of non-reduced pollen. In this work, the mechanisms responsible for the formation of 2n pollen in 28 dihaploid Solanum tuberosum x Solanum chacoense hybrids were studied. Four mechanisms were found: parallelspindles (ps), fused spindles (fs), premature cytokinesis-1 (pc-1) and premature cytokinesis-2 (pc-2). The ps mechanism was the most frequent, being found in 23 of the 28 assessed clones. The ps and fs mechanisms led to the formation of dyads by first division restitution (FDR), transferring about 80% of the heterozygosity to the progenies. The pc-1 and pc-2 mechanisms also led to the formation of dyads, but they were genetically equivalent to second division restitution (SDR), transferring only 40% of the heterozygosity to the progenies. Occurrence of FDR and SDR were shown to be associated in 12 clones, indicating that the clones can produce non-reduced microspores by more than one mechanism. However, only one mechanism is functional in a single pollen-grain mother-cell. Clones 9-2, 9-3, 9-6 and 15-15 are recommended for use in 4x x 2x matings. aBackcrossing aHybrids aPotatoes aSolanum chacoense aBatata aMelhoramento genético vegetal aProgenie híbrida aRetrocruzamento aClone híbrido aFitomejoramiento aHíbridos aPatatas aPlant breendig aRetrocruzamiento aSolanum tuberosum L1 aDAVIDE, L. C.1 aPINTO, C. A. B. P. tBrazilian Journal of Geneticsgv. 18, n. 3, p. 445-450, 1995.