01601naa a2200241 a 450000100080000000500110000800800410001910000200006024501020008026000090018252008670019165000160105865000180107465000140109265300230110665300280112965300150115765300310117270000260120370000230122970000170125277300900126910489952010-12-01       2006    bl uuuu     u00u1 u    #d1 aSALDANHA, C. W.  aIn vitro morphogenesis in zygotic embryos and leaf sheaths of Euterpe edulis Martius (Arecaceae).  c2006  aDifferent factors associated to somatic embryogenesis induction in Euterpe edulis were investigated in the present study. Zygotic embryos were inoculated in MS culture medium supplemented with 2.4-D (0, 30, 35, 40 mg L-1). 3 mg L-1 2iP, 0.5 g L-1 glutamine, 0.5 g L-1 activated charcoal; 30 g L-1 glucose or sucrose and gelled with 5 g L-1 Agar. Leaf sheaths were inociulated on MS culture medium supplemented with Picloram (72.3 mg L-1) or 2.4-D (66.3 mg L-1), 3 mg L-1 2iP, 0.5 g L-1 glutamine (0; 0.29; 0.58. 1.17 g L-1), 1.5 g L-1 activated charcoal and gelled with 2.5 g L-1 Phytagel®. Indirect somatic embryos were induced from zygotic embryos on culture medium with 40 mg L-1 2.4-D and 30 g L-1 sucrose. The highest percentage of callus formation on leaf sheaths occurred in MS culture medium supplemented with 1.17 g L-1 glutamine and 66.3 mg L-1 2.4-D.  aAgricultura  aBiotecnologia  aGenética  aCultura de tecidos  aEmbriogênese somática  aPalmiteiro  aReguladores de crescimento1 aMARTINS-CORDER, M. P.1 aSTEINMACHER, D. A.1 aGUERRA, M. P  tCrop Breeding and Applied Biotechnology, Londrinagv. 6, n. 3, p. 228-235, June 2006.