01788naa a2200205 a 450000100080000000500110000800800410001910000180006024501760007826000090025452011440026365000110140765300150141870000210143370000200145470000180147470000160149270000180150877300560152614891102018-05-30       2004    bl uuuu     u00u1 u    #d1 aBEHURA, S. K.  aA physically anchored genetic map and linkage to avirulence reveals recombination suppression over the proximal region of Hessian fly chromosome A2.h[electronic resource]  c2004  aResistance in wheat (Triticum aestivum) to the Hessian fly (Mayetiola destructor), a major insect pest of wheat, is based on a gene-for-gene interaction. Close linkage (3+or-2 cM) was discovered between Hessian fly avirulence genes vH3 and vH5. Bulked segregant analysis revealed two DNA markers (28-178 and 23-201) within 10 cM of these loci and only 3+or-2 cM apart. However, 28-178 was located in the middle of the short arm of Hessian fly chromosome A2 whereas 23-201 was located in the middle of the long arm of chromosome A2, suggesting the presence of severe recombination suppression over its proximal region. To further test that possibility, an AFLP-based genetic map of the Hessian fly genome was constructed. Fluorescence in situ hybridization of 20 markers on the genetic map to the polytene chromosomes of the Hessian fly indicated good correspondence between the linkage groups and the four Hessian fly chromosomes. The physically anchored genetic map is the first of any gall midge species. The proximal region of mitotic chromosome A2 makes up 30% of its length but corresponded to &lt3% of the chromosome A2 genetic map..  aInseto  aCromossomo1 aVALICENTE, F. H.1 aRIDER Jr. S. D.1 aSHUM-CHEN, M.1 aJACKSON, S.1 aSTUART, J. J.  tGenetics, Marylandgv. 167, n. 1, p. 343-355, 2004.