01951naa a2200169 a 450000100080000000500110000800800410001910000230006024500990008326000090018252013170019165000160150865000200152470000160154470000200156077302010158014715192009-11-25       2008    bl ---       0--  u    #d1 aSOSA-GÓMEZ, D. R.  aNew PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson.  c2008  aThe variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification.  However, the lack of information on this region in Nomuraea rileyi ha precluded the development of specific primers for PCR amplification.  Also, due to the high variability in this region, amplification is not possible with universal primers. Thus, to obtain sequences of the IGS region a  new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or E04, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer  product amplification and DNA bands produced by 10-mer amplification.  When DNA products were absent in the RAPO amplification and present in the combination IGS-10 mer amplification, these products were gel-purified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 681 to 1,025 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for Nomuraea rileyi.  aEntomologia  aNomuraea Rileyi1 aBINNECK, E.1 aMARIN, S. R. R.  tIn: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 41.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS, 9., 2008, Warwick. Abstracts... Warwick: Society Invertebrate Pathology, 2008