03110nam a2200229 a 450000100080000000500110000800800410001910000230006024501230008326001240020630000110033052023760034165000170271765000140273465000140274865000170276265300280277965300220280770000170282970000180284670000160286414679342020-02-27       2003    bl uuuu     u00u1 u    #d1 aSÓSA-GOMEZ, D. R.  aPhylogenetic relationships of entomopathogenic fungi based on mitochondrial SSU rDNA sequences.h[electronic resource]  aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlingtonc2003  ap. 54.  aMembers of the Clavicipitaceae include endophytes as well as pathogens of grasses, insects and parasites other fungi. The parasitic interrelationships imply intricate mechanisms for host recognition and specificity. Therefore it is expected that through the evolutionary history these interrelationships should be conserved and recorded as genetic information. Comparative DNA sequence studies can bring new insight on the patterns of host specificity within the Clavicipitaceae group and help clarify phylogenetic relationships among therm. Our purpose was to compare the sequences of the mitochondrial SSU rDNA fragments from several mitosporic Clavicipitaceae fungi (Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, M. flavoviride, Nomuraea anemonoides, N. cylindrospora, N. rileyi, N. viridulus, Paecilomyces amoenoroseus, P. fumosoroseus, P. javanicus, Tolypocladium cylindrosporum, and Verticillium lecanii) to infer phylogenetic relationships among them. Isolates of these fungi were obtained from the ARSEF and from Embrapa Soja culture collections (www.ppru.cornell.edu/mycology/insect.htm and www.cnpso.embrapa.br/pubonline.htm). The DNA was extracted from mycelia using a CTAB protocol and, after clearing and quantification, amplified with the appropriate primers. The amplified DNA was cleaned and sequenced in a ABI sequencer at the BioResource Center, Cornell University. Homologous sequences from the NCBI GenBank database, mostly from Cordyceps species, were included in the analysis. Two fragments, 393 and 429 bp were aligned by means of Clustal W and parsimony analysis was performed with Mega2 software; bootstrap analysis was done using 1,000 replicates. The most divergent fungus was T. cylindrosporum (ARSEF 963). Species of Metarhizium (M. anisopliae and M. flavoviridae) were closely related to Nomuraea, as observed by other authors when the ITS region was considered N. anemonoides clustered in the same group with B. brongniartii and B. bassiana. Paecilomyces javanicus, P. tenuipes, P. amoenoroseus and Cordyceps militaris clustered in the same group. It was not possible to obtain the complete sequences of N. cylindrospora and N. viridulus, but these species to be more closely realted to Metarhizium than to Nomuraea, thus implying that a revision of this group of genera is needed. N. rileyi seems to be a monophyletic group.  aMitochondria  aPhylogeny  aFilogenia  aMitocôndria  aFungo entomopatogênico  aSequência de DNA1 aHODGE, K. T.1 aHUMBER, R. A.1 aBINNECK, E.