03425naa a2200169 a 450000100080000000500110000800800410001910000230006024501240008326000090020730000120021649000370022850001800026552025430044570000210298877302460300914668392007-07-27       2004    bl uuuu     u00u1 u    #d1 aSOSA-GÓMEZ, D. R.  aCompatibility of agrochemicals with the entomopathogenic fungus Metarhizium anisopliae (DeuteromycotinabHyphomycetes).  c2004  ap. 295.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aThe entomopathogenic fungus Metarhizium anisopliae is a natural control agent of several beetles, Phyllophaga spp., (Scarabaeidae), Diabrotica speciosa, Maecolaspis sp. (Chrysomelidae) in soybean agroecosystems. The use of fungi as microbial control agents usually shows high variability, because they are highly influenced by environmental factors. The joint application with agrochemicals may help improving their potential as control agents. The synthetic substances could act as stressor agents and facilitate the action as infectious diseases. We studied the compatibility of insecticides and fungicides with M. anisopliae isolates. Agrochemicals were mixed at the recommended rates per hectare considering a application volume of 100 L ha-1. Recommended rates in 100 ml of sterile distilled were: Regent 800WGâ (fipronil, 25, 50, and 100 mg a.i.); Karate zeon 50CSâ (lambda-cyalothrin, 0.83, 1.66, and 3.33 mg a.i); MatchCEâ (lufenuron, 7.5 mg a.i); Certero SCâ (triflumuron 62.5 mg a.i.); Provado 200SCâ (imidacloprid, 69.3 mg a.i); Standak FSâ (fipronil, 10 mg a.i); Provado Duo SCâ (imidacloprid + beta cyfluthrin, 8.39 mg a.i); Dipterex 500CSâ (trichlorfon, 400 mg a.i); Dissulfan CEâ (endosulfan, 87.5 mg a.i); Actara 250WGâ (thiamethoxan, 37.5 mg a.i); Dimilinâ (diflubenzuron, 7.5 mg a.i); Previcurâ (propamocarb, 288.8, 433.2, and 577.6 mg a.i). Conidia of 12-14 days colonies of the isolates CNPSo-Ma12, CNPSo-Ma64, CNPSo-Ma136, CNPSo-Ma468 were produced on potato dextrose agar (PDA) medium. Conidia were exposed to agrochemical suspensions for four hours under gently vortex and suspensions were nebulized over a thin layer of PDA medium (with streptomycin) distributed on slides. Each treatment was replicated four times. Slides were incubated at 26±2 °C in wet chambers for 24 h in the darkness. Germinated and ungerminated conidia were counted (ca. 100) after 18 and 26 h interval under a contrast phase microscope at 400 x magnification. Data were analyzed with Sigmastat software using ANOVA, and means were compared with Student-Newman-Keuls method test at 5%. Agrochemicals ordered from the less to the most deleterious, considering percentages of conidial germination inhibition were, propamocarb, lambda-cyalothrin, fipronil (Regent800WG), imidacloprid, lufenuron, triflumuron, diflubenzuron, thiamethoxan, fipronil (Standak FS), imidacloprid+beta-cyfluthrin, trichlorfon and endosulfan. Possibly the eight first products can be used simultaneously with M. anisopliae to control the referred pests.1 aVIEIRA, I. H. L.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.