02470naa a2200193 a 450000100080000000500110000800800410001910000200006024500890008026000090016930000120017849000370019050001800022752015530040770000270196070000240198770000190201177302460203014668112007-07-27       2004    bl uuuu     u00u1 u    #d1 aCOSTA, N. P. da  aEvaluation of the alternative methodology for the tetrazolium test in soybean seeds.  c2004  ap. 226.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aThe study of fast methods in programs of quality control is basic upon the evaluation of the potential of a batch of seeds. Among these, the tetrazolium test has been considered as a promising tool, which have a high degree of reliability, especially for determining seed quality in the stages of harvest, drying, procesing and storage of seeds. The normal methodology establishes a period of 16h/25ºC for preconditioning before staining the seeds. However, this period can be considered long and often can delay the generation of the required information. This study had the objective of validating the use of a reduced period for preconditioning soybean seeds for the TZ-test. Forty soybean genotypes were used. Two preconditioning periods (4h and 6h) at 41ºC were tested and compared to the period of 16h at 25ºC. The following parameters were determined: seed moisture content, TZ-vigor, TZ-viability, mechanical damage, weathering and sting-bug damages. The preconditioning period of 6h/41ºC resulted in adequate staining of the seeds, allowing excellent interpretation of the results. This staining pattern was equivalent to the traditionally recommended period of 16h/25ºC and had a better precision than the treatment of 4h/41ºC. The 6h/41ºC preconditioning treatment increased seed moisture content to 27% or more, which is considered adequate for a good staining pattern. It was concluded that seed preconditioning for 6h/41ºC can be successfully applied to soybean seeds for the assessing of seed quality by the tetrazolium test.1 aFRANÇA NETO, J. de B.1 aKRZYZANOWSKI, F. C.1 aHENNING, A. A.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.