03062naa a2200205 a 450000100080000000500110000800800410001910000200006024501200008026000090020030000140020949000370022350001800026052020860044070000210252670000200254770000230256770000200259077302460261014667522007-07-27       2004    bl uuuu     u00u1 u    #d1 aCATTELAN, A. J.  aSoybean sudden death syndrome (Fusarium solani) controlled in greenhouse by inoculation with antagonistic bacteria.  c2004  ap. 71-72.  a(Embrapa Soja. Documentos, 228).  aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.  aThe soybean sudden death syndrome (SDS), caused by Fusarium solani f. sp. glycines (FSG), is a disease that has grown in importance in Brazil. Besides a few tolerant cultivars, no agricultural practice has been successful in reducing the impact of the disease. The present study aimed to evaluate the effect of soybean seed inoculation with bacteria antagonistic to FSG upon the control or attenuation of SDS symptoms. Eighteen bacterial strains, most of them Pseudomonas spp., were tested under greenhouse conditions. The strains were grown in trypticase soy agar, one tenth of the strength (0.1X TSA), at 28±1°C, for 24 to 48 h. The bacterial cells were harvested, suspended in 0.1M MgSO4 (pH 7,0) and the optical density adjusted for an absorbance of 0.55 at 600 nm. In each pot containing 3 kg of the A horizon of an Eutrorthox ("Latossolo Vermelho Perférrico eutrófico"), were sown six seeds of soybean cv. "BRS 156" inoculated with each one of the strains and 18 sorghum seeds with mycelia of FSG (3,2x105 CFU seed-1). For the inoculation, the seeds were immersed in the cell suspension of each strain or into sterile 0.1 M MgSO4 pH 7.0 (nonbacterial control). There was also a nonbacterial control with soil non-inoculated with FSG. Each treatment was replicated seven times in a completely randomized design. Fifteen days after the emergence, plants were thinned to two per pot. The plants were harvest at 30 days after sowing when the following evaluations were made: plant height, root and shoot dry weight and number of nodules. The data were analyzed by ANOVA and treatment means were separated by the Duncan's test (a=0.05). The presence of the fungus in the soil reduced the shoot and root dry weight in 16,5 and 17,8 %, respectively, when the bacteria were not inoculated. In the presence of the fungus and bacteria, some strains protected the plants and the symptoms were virtually absent. In those cases, the development of the plants were very similar to the ones in the control without the fungus. The best strains are currently being tested in field trials.1 aCOLOMBANO, L. P.1 aBETTI, A. F. F.1 aCUNHA, F. E. I. D.1 aFERRACIN, L. M.  tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.