03523nam a2200169 a 450000100080000000500110000800800410001910000230006024500990008326000610018230000090024350000180025252030350027065000240330565000150332965000090334414593972008-11-10       2000    bl uuuu  m   00u1 u    #d1 aASSUNCAO, M. da S.  aSoybean cyst nematode, Heterodera glycines, resistance genes in PI 8972 and PI 209332 soybean.  aUrbana: University of Illinois at Urbana-Champaignc2000  a52p.  aPh.D. Thesis.  aSoybean cyst nematode(SCN), Heterodera glycines Ichinohe, is the most serious disease of soybean glycine max(L.) Merr., in the United States and also is a serious pest of soybean on a world-wide basis. The nematode was first found in the United States in North Carolina in 1954 and now occurs in 30 states. Crop rotation plays an important role in controlling the nematode. Control also utilizes different cropping systems and resistant soybean cultivars to supress yield loss caused by H. glycines. A racetest was developed in early 1970´s to classify variability in the nematode and was expanded in late 1980´s to include 16 races. Eight races have been identified in the United States and in the North Central United States race 3 is the prevalent. Several plants introductions have been found with resistance to the most important races that occur in the soybean production areas in Asia, North America, and South America. The number of resistance genes in PI 89772 and PI 209332 conferring resistance to H.glycines race 3 is not well defined. Crosses of PI 89772 x 'Lee 68', PI 88788 x PI 89772, and Lee 68 x PI 209332 were made in the field and greenhouse. To verify that F1 plants resulted from the cross rather than selfing, simple sequence repeat molecular marker analysis was used to characterize F1 plants and their parents. Several F1 and F2 families from each cross, 98 F3 families from cross PI 89772 x Lee 68, 74 F3 families from cross PI 88788 x PI 89772, and 80 F3 families from cross Lee 68 x PI 209332 were tested with an inbred line of H.glycines developedon 88788. Approximately 8,000 individual plants growing in pots containing 200 cm³ of sterilized soil were inoculated with 4,010 eggs and J2/pot. Thity days after inoculation the number of females that developed on each plant was determined. Cluster analysis revealed sets of families with a low mean number of femalesand low variance, intermediate means and high variance, and high means witha low variance, indicating F3 plants came from, respectively, homozygous resistant, heterozygous or segreganting, and homozygous susceptible F2 plants. Thus, resistance classes were considered as quantitative parameters having different levels of resistance as opposed to only two classes, either or susceptible. Chi-square analysis of segregation of phenotypic data indicated two genes confer resistance torace of H.glycines. One gene acts as a major gene (Rhgx) and the other a minor gene (Rhgy) in conferring resistance of the parents PI 89772 (Rhgx1?Rhgx1?Rhgy1?Rhgy1?) PI 88788 (Rhgx2?Rhgx2?Rhgy2?Rhgy2/), and PI 209332 (Rhgx3?Rhgx3?Rhgy3?Rhgy3) to H.glycine race 3. The same genes may occur in PI 209332 as in PI 89772, but support for this hypothesis must be obtained by studying the cross PI 209332 x PI 89772. The same major (Rhgx) and minor (Rhgy) genes occur in PI 89772 (Rhgx1?Rhgx1?Rhgy1?Rhgy1?) and PI 88788 (Rhgx2?Rhgx2?Rhgy2?Rhgy2?). The phenotypic ratios obtained in this research indicate that epsitasis occurs between loci Rhgyx and geney.  aHeterodera Glycines  aNematóide  aSoja