01483naa a2200337 a 450000100080000000500110000800800410001910000180006024500840007826000090016252006240017165000100079565000090080565000190081465000200083365000240085365000110087765300120088865300110090065300130091165300150092465300200093965300130095970000140097270000210098670000270100770000170103470000180105170000140106977300620108313544111998-04-29       1995    bl uuuu     u00u1 u    #d1 aENGELMANN, F.  aFactors affecting the cryopreservation of coffee, coconut and oil palm embryos.  c1995  aThis paper describes the importance of various parameters which can significantly influence the cryopreservability of zygotic and somatic embryos of several recalcitrant and intermediate seed species (coffee, coconut, oil palm). Embryos should be used only when they are in an optimal physiological state as regards notably their maturity and metabolic status. Modifications of recovery conditions can greatly increase the survival rate of zygotic embryos. In the case of oil palm somatic embryos, pregrowth on medium with hith sucrose concentration is necessary to induce tolerance to desiccation and cryopreservation.  aCafé  aCoco  aCocos Nucifera  aCoffea Arábica  aCultura de Embrião  aDendê  aCoconut  aCoffee  aCoqueiro  aDendezeiro  aEmbrijo culture  aOil palm1 aDUMET, D.1 aCHABRILLANGE, N.1 aABDELNOUR-ESQUIVEL, A.1 aASSY-BAH, B.1 aDEREUDDRE, J.1 aDURAL, Y.  tPlant Genetic Resources Newslettergn.103, p.27-31, 1995.