02445naa a2200313 a 450000100080000000500110000800800410001910000200006024501500008026000090023050000110023952015380025065000150178865000290180365000100183265000140184265000150185665000230187165000130189465000130190765300220192065300130194270000180195570000230197370000180199670000180201470000180203277300810205013254362009-07-27       2003    bl ---       0--  u    #d1 aAZZOLINI, S. S.  aRhipicephalus sanguineus trypsin inhibitors present in the tick larvaebisolation, characterization, and parcial primary structure determination.  c2003  aCNPGC.  aBlood sucking  animals are a rich source of proteinase inhibitors mainly those  thayt interfere  in their  host hemostatic systems. The tick Rhipicephalus anguineus  is an ectoparasite of dogs and other animals. The aims of thos work ere the purification and  characterization of serine proteinase inhibitors present in R. sanguineus larvae (Rs TI). The inhibitores (RsTI) were isolated by affinity chromatography on trypsin-sepharose and ion exchange chromatographies in Resource Q  and Mono S columns. These Rs'TIs were separated in around 12 different  protein peaks, when they showed molecular masses between 8 and 18 kDa, by SDS-PAGE. Purified Rs TIs presented differences in the specificility for differen serine proteinases. s TQ2 was, better inhibitor than RsTIQ7 and RsTISS for neutrophil elastase, plasmin and HuPK with dissociation constants (Kii) of 1.3, 3.2 and 22nM, respectively. Other inhibitors sus as RsTIQ7, Rs TIS3, and affected neutrophil elastase and plasmin with Ki in the nM range The RsTIQ2, RsTIQ7, and Rs TIS5 amino acid sequence data allowed classifying them  as members of the  Kunitz-type serine proteinas inhibitor family, even though the RsTI role still unknown. Our present results showed that serine proteinase inhibitors from R sangguineus are similar to inhibitors from Boophilus microplus other hard tick species, suggesting a similar role of these inhibitors in hard tick species and also as a potential tool to generate or improve vaccine against different ectoparasites with an unique antigen.  aimmunology  aRhipicephalus sanguineus  aticks  aCarrapato  aImunologia  aInibidor de Enzima  aProtease  aTripsina  aEnsyme inhibitors  aTrypsina1 aSASAKI, S. D.1 aTORQUATO, R. J. S.1 aANDREOTTI, R.1 aANDREOTTI, E.1 aTANAKA, A. S.  tArchives of Biochemistry and Biophysics, New Yorkgn. 417, p. 176-182, 2003.