02434naa a2200409 a 450000100080000000500110000800800410001910000190006024501450007926000090022450000110023352013030024465000110154765000110155865000170156965000150158665000130160165000210161465000110163565000100164665000180165665000240167465000150169865300110171365300090172465300230173365300230175670000220177970000180180170000160181970000230183570000180185870000200187670000180189670000190191477300910193313242522009-02-20       2001    bl uuuu     u00u1 u    #d1 aMADRUGA, C. R.  aEvaluation of an ELISA for detection of antibodies to Babesia bigemin in cattle and it's application in an epidemiological survey in Brazil.  c2001  aCNPGC.  aAn indirect  enzyme-linked  immunosorbent assay (ELISA) using a crude antigen was evaluated  of its performance to detect Babesia antibodies. The sensitivity and specificity were 98,0% and 99,0%, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 Babesia bovis organisms. With regard to the comparison of ELISA and  indirect fluorescent antibody test. (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves sera. There was a good agreement between results shown by ELISA and IFAT with sera  from an enzootically stable area (K=0,61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (K=0,33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four  counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87,7 to 98,9%. Therefore, this ELISA with high sensitivity, specificity  and performance similar to IFAT can be employed in serological  diagnosis of B. bigemina.  aBrazil  acattle  aepidemiology  aimmunology  aPantanal  aBabesia Bigemina  aBovino  aElisa  aEpidemiologia  aImunofluorescência  aImunologia  aBrasil  aIFAT  aImmunofluorescense  aMato Grosso do Sul1 aMARQUES, A. P. C.1 aARAUJO, F. R.1 aMIGUITA, M.1 aCARVALHO, C. M. E.1 aARAUJO, F. S.1 aUMAKI, A. C. S.1 aCROCCI, A. J.1 aQUEIROZ, R. A.  tPesquisa Veterinaria Brasileira, Rio de Janeirogv.21, n.2, p.72-76, abr. / jun. 2001.