02566nam a2200649 a 450000100080000000500110000800800410001902000180006010000130007824500900009126000220018130000090020349000350021252009480024765000200119565000200121565000220123565000130125765000140127065000230128465000280130765000130133565000150134865000180136365000080138165000170138965000160140665000140142265000240143665000220146065000160148265000140149865000130151265000110152565000220153665000200155865000150157865300090159365300170160265300140161965300210163365300230165465300150167765300270169265300260171965300220174565300250176765300100179265300180180265300110182065300090183165300100184070000180185070000160186870000160188470000160190012922801997-11-26       1997    bl uuuu     u0uu1 u    #d  a92-9043-323-X1 aKARP, A.  aMolecular tools in plant genetic resources conservationba guide to the technologies.  aRoma: IPGRIc1997  a47p.  a(IPGRI Technical Bulletin, 2).  aThe need for improved genetic resolution; A brief review of the basic techniques; Basic tools; Category 1: non-PCR based methods; Restriction fragment length polymorphism (RFLP) analysis; Variable number of tandem repeats (VNTRs); Category 2: arbitrary (or semi-arbitrary) primed techniques; Multiple arbitrarily primer PCR (MAAP) techniques; Amplified fragment length polymorphism (AFLP); Category 3: site-targeted PCR; Sequence-tagged microsatellites (STMS); Variations or combinations of the basic techniques; Using the different techniques; The basic technologies; Analysis of molecular data; Application of the techniques; Category 1 techniques: Non-PCR-based techniques; Category 2: arbitrary (or semi-arbritrary) primed/or multi-locus profiling techniques; Category 3: site-targeted PCR sequence techniques; Pre-screening and combinations of techniques; A framework for selection appropriate techniques; Using the decision-making chart.  aelectrophoresis  agenetic markers  agenetic resources  agenetics  agermplasm  amolecular genetics  aplant genetic resources  aresearch  atechnology  aConservação  aDNA  aEletroforese  aEquipamento  aGenética  aGenética Molecular  aGenética Vegetal  aGermoplasma  aIsoenzima  aPesquisa  aPlanta  aRecurso Genético  aRecurso Vegetal  aTecnologia  aAFLP  aConservation  aGene bank  aGenetic resource  aGenetics resources  aIsoenzymes  aMarcadores moleculares  aMelhoramento genetico  aMolecular markers  aMolecular techniques  aPlant  aPlant genetic  aPlants  aRAPD  aRFLPs1 aKRESOVICH, S.1 aBHAT, K. V.1 aAYAD, W. G.1 aHODGKIN, T.